FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell93.35100000095b33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell93.35100000095b33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503489
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25240.16787618665650364No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG16480.10961170982960301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT57350.021.852112
CGATCTT15450.021.844115
CCGATCT57300.021.8319134
CTTCCGA57700.021.8091531
CGATCTG20550.021.678295
CGATCTA9350.021.6572115
TCCGATC58650.021.367753
GTCCGCT600.006512351318.74952920
TTGTCGG1851.6370905E-1118.24278522
CTATCCG1002.0477599E-517.9995489
CCTAATA2500.017.099572
AACGCGC2005.0931703E-1116.87457542
GCGATTG1752.6902853E-916.7149827
GTATATA5300.016.1402591
GATCTGG8650.015.86665256
GATCTAG4400.015.8518746
GATCTTT12650.015.651786
TTAACGG2750.015.54609935
CGATTGC1907.998096E-915.39537428
ATAATAG8350.015.089443
Produced by FastQC (version 0.11.2)