FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell92.35100000095b19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell92.35100000095b19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625595
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA97560.37157291966201944No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC60240.22943370931160367No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT56960.21694130282850174No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG55280.21054275316642512No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA46390.17668376120460316No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT41390.157640458638899No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT38400.1462525637046079No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC37730.14370076116080355No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC36570.1392827149655602No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA35840.13650239279096738No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC29960.11410746897369929No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTA29270.11147949321963212No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTG28930.11018454864516423No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGG28800.10968942277845593No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGAT28210.10744231307570284No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATA27630.10523328997808115No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCA27630.10523328997808115No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCT27560.10496668374216131No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT26980.10275766064453962No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCT26780.10199592854191145No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAA26610.1013484562546775No Hit
TAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGA26520.10100567680849483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG26000.023.6239815
TTCCGAT67950.022.7155482
CTTCCGA68000.022.711391
TCCGATC68350.022.5817533
CCGATCT69800.022.1122264
CGGTACC6900.021.19473645
CGATCTT17700.020.210995
ATCTGCG3850.019.869277
CGATCTA11450.019.6498075
TAACGGC7500.019.50027536
CGATCTC17950.019.3027885
CGGTAAG1051.446293E-619.28671828
TTAACGG7700.018.9937735
ACCGCCT7950.018.6784412
CGCGGTA8000.018.56169943
TGCGATA851.2055882E-418.52861212
GGCACCG8350.018.0531129
GTATTAG14250.018.0101991
GCCGTAT750.001110777517.99990839
ACCGCGC750.001110919417.99956718
Produced by FastQC (version 0.11.2)