FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell91.35100000095afe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell91.35100000095afe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104122
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27570.1310285240114404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG19200.023.6716385
TCCGATC57600.021.9919663
CTTCCGA57500.021.8877371
CCGATCT57950.021.781494
TTCCGAT58250.021.669312
CGATCTT19300.021.2174035
CGGATCG754.604479E-521.00028837
CCCAACG1109.9975296E-820.45385421
TATAACG600.00651352318.7493677
CGATCTA8650.018.4680235
CGTTGTA1253.8373764E-717.99981926
TACGCAA1205.178883E-616.8744310
ATCTGCG2600.016.4417517
CGATCTC15250.016.2293455
GATCTTT19450.015.963856
GTATTAA6200.015.6147021
CGCTAGA5000.014.40019937
CGGGCTA1251.3814322E-414.39985641
TAGGACC7500.014.3998554
CGCGCAA3000.014.24951921
Produced by FastQC (version 0.11.2)