FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell90.35100000095ad4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell90.35100000095ad4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704279
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA18740.2660877294367715No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC11890.16882513890091852No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT10930.1551941773075727No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA10540.149656599160276No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG9840.13971735633179463No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT8230.11685709782628759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG450.001226308424.99921239
TCTGTCG651.5230349E-524.2300038
CGATCTG8800.022.7565785
CTGTCGC602.4667193E-422.499299
CGCATCG500.002264160422.4992913
CGATTAC702.7017311E-522.4992945
GCCGCGA500.002264160422.4992945
ATGGTCG500.002264160422.4992936
CCGTTAA1056.101436E-821.42789516
TAGCGGA754.600502E-520.99933617
TTCCGAT25400.020.8192272
CCGATCT25300.020.8110964
TCCGATC25500.020.7361133
CTTCCGA25750.020.46631
CGATCTT7200.019.6882765
TTAGGCG600.006509235618.74940936
CGATTAT600.006509235618.74940925
CATCGTT600.006509235618.74940928
TCGTAGC851.2038918E-418.52882814
GGTACCG1253.826426E-718.000716
Produced by FastQC (version 0.11.2)