FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell9.351000000950a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell9.351000000950a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723420
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27450.15927632266075595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA36300.027.4754351
TCCGATC36350.027.233653
TTCCGAT36600.027.1090982
CCGATCT36800.026.7172184
CGATCTG14450.026.624775
CGATCTT11150.023.8102915
CGATCTA4700.023.45615
CGATCTC11500.020.1510185
CGGTACT2900.019.39656638
CGAGCGT600.00651285318.74946619
GGTACTA3400.017.86765939
TTGCGGT3350.017.46320535
GATCTTT9100.017.059466
CGGTACC2005.0931703E-1116.87403145
TTAACGG2159.094947E-1216.74468235
GTATTAG6250.016.5701071
CCTTGCG3550.016.4798433
GTATTAT3400.015.8920461
TCTTCCG15950.015.0931795
AGCGAAC2101.8535502E-915.00044427
Produced by FastQC (version 0.11.2)