FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell89.35100000095aba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell89.35100000095aba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006830
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA16580.16467526792010567No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC10780.10706872063804217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG13000.028.5566845
CGGGTAT406.166882E-428.12400615
TTCCGAT31350.025.3352932
CTTCCGA31600.025.0798441
TCCGATC31650.025.0228143
CCGATCT31800.024.7632774
CGATCTA4400.023.0105515
GATCTGG7100.019.6471946
CGATCTT9700.019.2518985
GATCTGT4350.018.1028086
ATCTGCG1507.2650437E-917.9993657
TTATACC1053.1127463E-517.1422524
GGCGTTA1358.582956E-716.66607942
CGATCTC8000.016.5931645
CGAGCGA1652.357956E-816.36305845
TTATGCG2351.8189894E-1216.2768338
TATGCGG2351.8189894E-1216.2768339
TCTTCCG14850.016.060045
ATCTGGG5250.015.8565837
GTATTAG3700.015.8212521
Produced by FastQC (version 0.11.2)