FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell88.35100000095a91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell88.35100000095a91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440282
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.24620584080203142No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT8130.18465438060152356Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG7740.17579642138447632Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.14854116225510014No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5200.11810612289396341No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG207.028888E-444.99954629
TACCCAC250.00210580435.9996385
GAATAAT300.005132685430.0133321
CTATACT300.005144103429.99969728
TACACGT300.005144103429.9996972
CGCCCCC608.177194E-626.24973542
ATTCTAT450.001222182225.011111
TGTATAA450.001225424624.9997486
GTATAAT450.001225424624.9997487
CTCTCTA450.001225424624.99974828
TTGTACT551.3664244E-424.5452084
CACTATC551.3664244E-424.54520810
GTACTGA651.5210964E-524.2305266
ATGGGTC500.002262540422.49977312
ACTATCG602.4643244E-422.49977311
TTCACTA602.4643244E-422.4997738
TCACTAT602.4643244E-422.4997739
GAGCATC500.002262540422.49977333
TCTTGTA754.59471E-520.9997882
TATCGGT654.232797E-420.76902213
Produced by FastQC (version 0.11.2)