FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell87.35100000095a77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell87.35100000095a77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927850
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA15350.16543622352750983No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC9840.10605162472382389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG300.00514780829.9987412
CGATCTA3950.025.0622435
TTGCGGT1550.024.67771535
TTCCGAT27750.022.053132
CTTCCGA27750.021.9898261
TCCGATC27900.021.9345633
CCTTGCG1750.021.85740533
CCGATCT28250.021.5831664
CGATCTT8750.021.3419635
GGTACTA1800.021.25025439
CGGTACT1850.020.67592238
CGGGTAC1009.054693E-720.24915115
CGATCTG10450.020.2383865
CTTGCGG2100.019.28594634
TCTATCG1102.2547683E-618.40931131
TACGACG1701.8299033E-917.2051625
AACCGCG2005.0931703E-1116.87520241
CGGTACC1205.1729985E-616.87429245
GTATTAG3750.016.8128851
TAGTGCA1104.63763E-516.362954
Produced by FastQC (version 0.11.2)