FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell86.35100000095a5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell86.35100000095a5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347703
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA39640.29413008652499845No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC25970.1926982428621143No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT22120.16413111790950974No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG20820.1544850757177212No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA19850.1472876442361559No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT16570.12294993778302786No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT15260.11322969526668711No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC14240.10566126216236069No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA14120.10477085826773407No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC13830.10261904885571971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG15000.024.2988385
TTCCGAT38300.023.0284732
TCCGATC38500.022.9664343
CCGATCT38750.022.7602024
CTTCCGA38850.022.7193161
CGGTACC3200.022.49892445
TTAACGG3400.021.83881235
CGATCTA4900.021.58065
GAATCGG1700.021.17545722
CGATCTT10450.020.6688675
TCGAATT1653.6379788E-1220.45432740
TAACGGC3750.019.80052436
ACCGCCT3750.019.79905312
CGTAGCG1850.019.4585315
TCGTTAA1757.2759576E-1219.28479212
AATCGGG2350.019.14873123
GTATTAA3450.018.9268841
CCGGTAG600.006512863618.74910413
TCCGGTA600.006512863618.74910412
GCGGAAT1951.8189894E-1218.46065519
Produced by FastQC (version 0.11.2)