FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell85.35100000095a34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell85.35100000095a34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675687
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA71300.26647361967225613No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC48310.1805517610991121No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT40350.1508023920585629No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG40100.14986805257864616No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA34950.13062065929236116No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT29600.1106257944221428No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT29070.1086449947247193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA71900.022.6724931
TTCCGAT72200.022.5929932
TCCGATC72150.022.5774673
CCGATCT72050.022.483894
CGATCTG27550.022.3770035
CGATCTT20800.021.742315
CGGTACC4550.021.2628745
CGATCTA9800.021.1219845
ATGCGCC600.006514235418.74923711
CGATCTC19300.018.3027065
TTAACGG5350.018.08438935
TAACGGC5550.017.432736
AACGGCC5600.017.27705237
GATCTTT19600.016.8746286
CGCGGTA5800.016.68035743
CGCCGTA1501.4248508E-716.49994525
TCGAGTA1257.633997E-616.19994741
ATCTGCG3500.016.0707747
TCGGTAA850.002566416515.882327
CGGTACT6700.015.78352938
Produced by FastQC (version 0.11.2)