FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell84.35100000095a1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell84.35100000095a1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567984
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA33130.2112904213308299No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC19070.12162113899121418No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG18790.11983540648373964No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT16950.10810059286319247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG500.002264807522.50028637
CGATCTT13800.021.0315365
TTCCGAT47100.019.776062
CCGATCT47350.019.6716484
CGATCTG16900.019.5700075
TCCGATC47750.019.5068593
CTTCCGA48650.019.2970391
CGTAGCG1102.2581462E-618.40815215
CGATCTA8000.018.2803175
TAGCCGC1002.0483069E-517.9990812
CGGTACT3050.017.70457838
CGCTAGA901.8660421E-417.50022337
AACGCCG901.8669524E-417.4991075
TAACGCC1053.1149553E-517.1419834
ACGACGG800.001711804916.87413817
GATCTTT11700.016.7299166
CGGTACC3500.016.71343445
GTACTAG1358.534753E-716.6764531
CGCATCG952.8212744E-416.57810213
TAGCGCG1104.6362078E-516.3648929
Produced by FastQC (version 0.11.2)