FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell83.351000000959ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell83.351000000959ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734793
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA51570.29726889605849227No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC29590.17056790060831464No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG28920.16670576835391887No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT26170.15085373298139892No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA22600.1302749088796185No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT21240.122435356840845No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT20580.11863086835144021No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC18300.1054880899334964No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC17930.10335527062883007No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC17490.10081894496922687No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA17420.10041543861429002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG855.035541E-621.17603543
TATACCG1703.6379788E-1219.8531076
GGACCGT706.985041E-419.2858736
CGGGTAT706.986396E-419.28531815
CCGGTAG706.986396E-419.2853189
GTACGTA1403.090463E-919.28531813
CGGTACC3100.018.8705845
CGATCTT23050.018.8396365
CGGTAGC851.2050793E-418.52903210
TTAACGG3200.017.57776535
ATCTGCG2200.017.3860077
TAACGCC1305.78626E-717.3078374
GCGAACT2600.017.30733928
AAATGCG1053.1136777E-517.1435
GGGACGA1053.114448E-517.14250612
GACGATT1053.114448E-517.14250614
GTAGCGC1459.674841E-817.0691073
CGGTACT5450.016.92625838
CGCTATC800.001711554216.87465512
CTTGCGG5600.016.87465334
Produced by FastQC (version 0.11.2)