FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell82.351000000959c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell82.351000000959c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847694
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA48250.261136313696965No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC25940.14039121196475174No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG25480.13790162223831437No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT24970.1351414249329164No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA20640.11170680859492968No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT19770.1069982367210155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGTA1204.5292836E-1022.4992149
CGATCTG23700.021.6459875
CGATCTT18350.020.8450895
TCCGATC67050.020.0339433
TTCCGAT67200.020.0227072
CCGATCT66800.019.9741864
CTTCCGA70000.019.4531211
CTAGCGT600.006511236518.7503594
GATCTGG10050.017.9107916
CGATCGC2450.017.4483715
TACGACG1305.7861143E-717.3080255
CGATCTA9800.017.2197195
CACGAGG4450.017.1904114
CGAGGGT4200.017.1422616
ACGAGGG4300.016.74360315
AGGCGGT3700.016.41923539
GATCTTT16200.016.2503136
TACGCCT1257.633722E-616.1994347
CCCAACG1257.633722E-616.19943421
TAATACG1401.2554519E-616.071744
Produced by FastQC (version 0.11.2)