FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell81.351000000959af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell81.351000000959af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555417
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA29010.11352354625487739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG32900.022.841425
CTACGCG500.00226566622.4994819
TCCGATC88700.021.2565573
TTCCGAT89600.021.0945032
CCGATCT89600.021.0932644
CTTCCGA91250.020.9238451
CGATCTA13950.020.3221135
CGATCTT26600.019.8773615
GTATTAA7250.018.6333871
CGGGTAT1601.6165359E-816.8746115
CGGTCGA800.001711882816.8746120
GTATTAG10500.016.2968181
GTATATA7200.015.9483661
TACCGTC4400.015.8519097
TGTGCGA1004.170259E-415.74963710
CGTCGAC1004.170259E-415.74963742
GATCTGT13250.015.6222816
GACGGTA5050.015.1481668
ACGACGG5100.014.9996556
GATCTTT24650.014.9692286
Produced by FastQC (version 0.11.2)