FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell80.35100000095985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell80.35100000095985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570214
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA42000.26747946458253463No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC22870.14564893702387063No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT21390.13622347017667655No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG20130.1281990862392005No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA17410.11087660662814112No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT15980.10176956771497388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGT801.0544318E-725.3121976
CGATCTT12500.022.319735
TTCCGAT45150.021.4781482
TCCGATC45050.021.475883
CCGATCT45100.021.452074
CTTCCGA46550.021.1336351
GACTCGA654.2395265E-420.76898211
CGGTATA550.003937443720.454324
CGATCTA7500.020.0997585
TACGACG1351.9663275E-919.9997625
CGGTACC3100.019.59653945
CGATCTG17000.019.3232985
TTAACGG2950.019.06756835
ACCGCCT3250.018.69208312
ACGACGG1454.776666E-918.6204646
CGACGGT1507.2723196E-917.9997837
GGCCGCG4200.017.6783640
CGCTAGA2800.017.67835837
CGATCTC12050.017.5516575
CCGCTAG2850.017.36821236
Produced by FastQC (version 0.11.2)