FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell8.3510000009508e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell8.3510000009508e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209187
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA62400.2824568495107023No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC37530.1698814993932157No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG37120.16802561304226396No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT33420.15127737036294348No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA27900.1262908029062275No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT25680.1162418572986352No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT25070.11348066053258506No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC23980.10854671877029876No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA22380.10130423544951152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG19600.025.3694115
TTCCGAT49700.025.3063582
TCCGATC49700.025.2610873
CTTCCGA50400.025.195771
CCGATCT50200.024.9198424
CGATCTT16050.023.6911355
CGATCTA7600.022.2035225
CGGTACT6200.019.95929538
GTGCGCG706.987159E-419.2853459
CCTTGCG6600.019.09097733
TACCGTC951.3169465E-518.9470067
GGTACTA6800.018.85993439
GCCGGTT600.00651222118.75006729
ATCGTAC600.006513071718.74964110
CGATCTC11550.018.3113385
CCGAACG750.001110806617.99965513
TTGCGGT6950.017.80541435
TGCGGTA7600.017.76281736
GTATTAG9550.017.6822261
CTTGCGG7000.017.67863334
Produced by FastQC (version 0.11.2)