FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell79.3510000009596b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell79.3510000009596b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408293
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA28520.11842412862554516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG24700.023.5019265
TCCGATC64300.023.40973
TTCCGAT65300.023.0856632
CTTCCGA65600.022.9915431
CGATCTA9200.022.9890355
CCGATCT65300.022.9822944
CGATCTT19150.021.3837225
GTATATA7400.017.339651
CGATCTC18350.016.7982965
TCGCCTA1803.914465E-916.25026933
CGCCTAT1855.627953E-915.81107334
GTATTAG8000.015.7577871
TCAACGC2651.8189894E-1215.28295525
CGTTAAT1209.7181866E-515.0074161
GTATAAA5600.014.8734221
CTCGTTA5200.014.71117211
GATCTTT18150.014.6280386
GTATTAA6950.014.5755481
ATTCGTA5250.014.57136824
AAGCTCG8550.014.21046724
Produced by FastQC (version 0.11.2)