FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell78.35100000095942.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell78.35100000095942.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991431
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA13510.13626767773047244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG9850.025.5833845
CGTAGCG551.368621E-424.5438615
CGATCTA4600.024.4561635
TCCGATC27300.023.9831683
TTCCGAT27450.023.7701452
CCGATCT27550.023.6838654
CTTCCGA27950.023.5154061
CGATCTT7050.021.063525
CGTCGTA1251.7678758E-819.79871410
ACCGTCG1403.088644E-919.2844628
TAAGACG600.006510426318.7497275
GTATTAA2350.018.1985681
AATCGGG750.001109810218.00064523
TCGCCGG1002.047319E-517.99882917
CCGTCGT1507.2668627E-917.9988299
ATAGCGT1153.4481764E-617.6084386
TAGCGTA1153.449837E-617.6075527
CGGTACC1153.449837E-617.60755245
GCGTATA1153.449837E-617.6075529
ATAGGGG2950.017.5421163
Produced by FastQC (version 0.11.2)