FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell77.35100000095935.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell77.35100000095935.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042884
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA29170.14278833257297036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTCG551.3689687E-424.5447736
CGATCTG22450.023.6519075
TCCGATC59050.022.9756573
TTCCGAT59450.022.9357242
CCGATCT59450.022.7453734
CGATCTA8600.022.4993765
CTTCCGA60700.022.471661
CGATCTT17400.020.6890815
TACCGTC2200.020.4534787
ACCGTCG2150.017.7897688
CTAGCGT800.001711778116.874534
GGCGTTA2950.016.77878442
CGCTAGA5200.016.44265637
GTATTAG8650.016.1334321
CGATCTC16650.016.0806355
CTATCGC2101.07320375E-1016.07216332
TTAACGG2101.07320375E-1016.07216335
TCCTATA2009.295036E-1015.7503342
TAGACTG2009.295036E-1015.7495625
GCATTCG5150.015.72733422
Produced by FastQC (version 0.11.2)