FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell76.3510000009591b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell76.3510000009591b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860753
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA33340.11654274241781797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG30300.024.2812275
TTCCGAT75750.023.1377072
TCCGATC76100.023.0608583
CCGATCT76900.022.7916934
CTTCCGA77500.022.6903571
CGATCTA12700.020.3732175
CGATCTT19600.019.8589135
TGCGATA706.986329E-419.28596926
CCGGTAA600.006514943718.7489388
CGATTAC1205.181324E-616.87404345
CGATCTC22600.016.7250085
AACCGCG7500.015.30020141
CGGTACC3400.015.21972645
TATTAGA7250.015.2063012
TTAACGG3300.015.00046135
CGGGTAT1654.2088504E-714.9991515
CGTCGTA2850.014.9991510
GATCTGC17050.014.9114386
ATCTGCG4400.014.8287057
CTCGTTA4450.014.66208911
Produced by FastQC (version 0.11.2)