FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell75.351000000958e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell75.351000000958e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451841
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA26770.18438658227726037No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC15860.1092406124362103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC3900.020.1916283
CGATCTG21300.019.3303345
TCCGATC55350.019.2676453
CCGATCT55550.019.0767654
TTCCGAT56450.018.8523332
CTTCCGA56900.018.831591
TATCGAG600.00651256718.7493710
CGATCTT15550.018.6649675
CGATCTA8650.018.207485
TACCGTC2400.017.8119017
CGTTATT1651.2314558E-917.72667544
TCTATAC2053.6379788E-1217.5603853
GGCGTTA1853.074092E-1017.02645542
GCGTTAT2005.0931703E-1116.87443243
ATAATAC5100.016.7641413
CCGGGTA952.8207203E-416.57838814
ATACCGT2950.016.016416
ACCGTCG2550.015.8818198
GATCTGA8350.015.6282176
TCTACGC1301.10714445E-515.5763993
Produced by FastQC (version 0.11.2)