FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell74.351000000958c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell74.351000000958c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491473
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA45160.1812582355899502No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC27850.11178126353366062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG26950.023.1263245
TCCGATC70850.022.8336143
CTTCCGA71400.022.7632271
TTCCGAT71700.022.5633752
CCGATCT71700.022.500164
ACGTCCG1104.0599843E-922.49925643
CGATCTT20000.021.7126545
CGATCTA11050.019.5476515
CGATCTC19150.018.2116185
GTATTAG10550.017.0699561
CGGTACC4250.016.94027745
ATCTGCG3250.016.6145027
TTAACGG4000.016.31261635
TTAGACG1401.256305E-616.0712239
ATCGATA1004.1705227E-415.7494823
CCGCGCA1004.1711595E-415.7491659
GATCTTT16600.014.6386596
GGACCGA1553.6209767E-614.51623256
CGCCTAT2950.014.49279234
TATTAGA8400.014.4646792
Produced by FastQC (version 0.11.2)