FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell73.351000000958a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell73.351000000958a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264695
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23020.10164724168155094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT21150.022.3400975
CGATCTG27000.021.4996835
TCCGATC73700.021.3395773
CCGATCT74100.021.2243844
TTCCGAT75150.020.8978942
CTTCCGA76400.020.7745131
CGATCTA9850.019.4159565
GTCGCTA750.001110785417.99973538
TACCGTC4150.017.3487597
ACCGTCG3800.017.1704228
GATCTTT19950.016.4659236
CGTCGTA4050.016.11051810
CTACCGA3600.015.62511525
TATATAC6800.015.5512413
GTATTAA7100.015.5372031
CGAGCCT3650.015.41141229
CGATCTC21850.015.3430235
CCTACCG3700.015.20281424
TATCCCG1351.5831185E-514.9997795
TACCGAG3800.014.8027426
Produced by FastQC (version 0.11.2)