FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell72.35100000095893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell72.35100000095893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634496
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA36250.1375974759498591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG24350.023.8386695
TCCGATC61000.023.6054023
CCGATCT60850.023.552674
CGATCTA8750.023.3988535
TTCCGAT61850.023.2809942
CTTCCGA63650.022.7774091
CGATCTT17250.022.1728295
ACGACGG4250.017.9991196
GGCGTTA3200.017.57759942
TACGACG4150.017.3485495
CGACGGT4500.016.9991687
CGGTACT5000.015.75012638
GCGTTAT4000.015.749228543
CGATCTC17400.015.5164825
ATCTGCG2900.015.5164817
CGTTATT4100.015.36510344
GACGGTA4750.015.1571538
TTTAGCG900.00375401415.00097534
TACCGTC3450.014.9992677
ACCGTCG3500.014.7849918
Produced by FastQC (version 0.11.2)