FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell71.35100000095879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell71.35100000095879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660860
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19020.2878067972036437No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG12570.1902067003601368No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC10860.16433132584813728No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT10790.16327209999092093No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA8180.12377810731471113No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT8050.12181097357988077No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT7480.1131858487425476No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC7340.11106739702811488No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC7330.11091607904851254No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC7170.10849499137487516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAG207.031282E-444.9997728
TCTACCG250.002106514335.9998173
CCCGCGA406.1635295E-428.12485720
CGGTACC1550.026.128945
CGCGAAG450.001226038824.99987422
CGTCGGC450.001226038824.99987441
CCGCGAA450.001226038824.99987421
CGTTGTA702.7008527E-522.49988721
CCGTCGG500.002263665722.49988740
TTAACGG1800.022.49988735
CGTCGTA500.002263665722.49988710
CCCGTCG602.466054E-422.49988639
GGCCGCG2050.021.95110940
TAACGGC1850.021.89178336
CGCGGTA1900.021.31568343
ATACCGC754.5990102E-520.9998953
AGCGCCC550.003934909620.45444135
GTCGGCA550.003934909620.45444142
CGATCTG8150.019.87725
CCGATCT20750.019.8432734
Produced by FastQC (version 0.11.2)