FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell70.35100000095850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell70.35100000095850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879164
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA92330.3206833650323497No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC52780.1833171017698193No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG50760.17630117631367995No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT48300.16775702947105478No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA40670.1412562813372215No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT37050.12868318720295197No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT35110.12194512018071912No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC33620.11677000684921039No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA31020.10773960774724885No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC30560.10614192175228644No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC29160.1012793991589225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG31450.021.461865
TTCCGAT81600.021.0929852
TCCGATC82200.020.9390223
CGATCTT21300.020.9147365
CCGATCT82150.020.896994
CTTCCGA83800.020.7111131
TTAACGG5750.019.56586635
CGGTACC5850.019.23007245
TAACGGC6250.018.36061136
ATCGTAC1002.0480966E-518.00028425
CGATCTA12200.017.5198575
CGCGGTA6550.017.51844843
CGGACGT901.8664339E-417.50058236
GTATTAG14000.017.365561
CGATCTC22800.016.874395
AACGGCC6950.016.51076537
ACCGCCT7150.016.36304312
ATCTGCG3150.015.7137167
GATCTGG15400.015.632556
GGCCGCG8350.015.62871740
Produced by FastQC (version 0.11.2)