FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell7.35100000095071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell7.35100000095071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486385
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA46950.18882835924444527No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC29820.11993315596739845No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT26350.10597715156743626No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG26290.10573583737031875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG18750.028.3191785
TCCGATC46150.027.6915043
CTTCCGA46500.027.5953831
TTCCGAT46600.027.3763662
CCGATCT47200.027.0754834
CGATCTA7400.025.8438435
CGATCTT14500.022.0338425
CGCGGAA807.565698E-519.68811433
GGATCGA600.00651367118.7494546
TACCGTC2450.018.3668147
TTAACGG3800.018.35546935
CGGTACC3900.017.88409445
CGATCTC13650.017.472025
CGTCGTA2650.016.98063910
CGGTACT4800.015.937036538
TAACGGC4250.015.88221136
TCTTCCG21600.015.4162195
GATCTGC11150.015.3358776
ACCGCCT4550.015.32922612
ATACCGT3250.015.2303276
Produced by FastQC (version 0.11.2)