FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell69.35100000095829.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell69.35100000095829.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810545
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA8890.10967928986052596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC20700.024.7836633
TTCCGAT21050.024.264692
CCGATCT21150.024.1499614
CTTCCGA21450.024.0338631
CGATCTA3150.023.572435
CGATCTT6050.023.4307485
CGATCTG8250.021.2736325
GTATAAC654.221441E-420.7803691
CGATCTC5750.020.7400135
ACACGCT600.006509598318.7496419
AATTCGA800.00171047716.87467627
CGTCGTA800.00171047716.87467610
ATATTAG2650.016.1407281
GACTGTA1552.0642801E-715.9674359
TATACCT5000.015.7506695
TTAGCTA1004.1657587E-415.74969825
GATCTAA1156.7475696E-515.6605681
GATCTCG1907.9780875E-915.3953916
GTATAGA1351.5724125E-515.0080441
GATCTAC2253.0377123E-1015.0006376
Produced by FastQC (version 0.11.2)