FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell68.3510000009580f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell68.3510000009580f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999479
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA13600.13607089293521923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA300.00514716529.99973341
GTCGACG406.165897E-428.1247543
TTAACGG856.4774213E-926.47167835
CGATCTG10500.025.2842245
CTTCCGA26300.024.0517441
TCCGATC26350.023.6513583
CCGATCT26400.023.6065654
TTCCGAT26400.023.6065652
CGATCTA4600.022.9877765
CCGATTG500.002263863622.50092736
GTATTAG3700.021.2942491
CGATCTT6400.021.0925085
CGATTGG550.003936386720.45436337
CGGTACC1109.981886E-820.45436345
TATCCCG550.003937527520.453345
TATTAGC3000.020.2488082
GTCCGAT600.006508370418.7507734
GCGTTAT1202.4960718E-718.74983443
TTTCGGG1951.8189894E-1218.46137422
TATCGAT750.001110151517.9998442
Produced by FastQC (version 0.11.2)