FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell67.351000000957e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell67.351000000957e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940626
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA13510.14362775428278612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT6500.023.5370455
TTCCGAT24800.022.5893652
TCGATAA500.00226439922.49984224
TCCGATC25200.022.4093653
CTTCCGA25100.022.3348081
CCGATCT25450.022.2776374
CGATCTG10850.020.943445
CCCGCGA1009.0565663E-720.24878120
CGATCTA3700.020.0663595
GTCTAGG1302.7506758E-819.050481
CCGTCGG1202.4942528E-718.75086440
CCGGTAG1205.1740535E-616.8739839
CGATCTC6850.016.7508165
GATCTTT4900.016.5296176
TACGACG1257.627572E-616.1990245
GTATTAG4050.016.121281
ACGCTTT1401.2548917E-616.07046144
ATTCGTA2251.8189894E-1115.99988624
GCATTCG2251.8189894E-1115.99903722
CGAGTTC1004.1679453E-415.74905114
Produced by FastQC (version 0.11.2)