FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell66.351000000957ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell66.351000000957ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269531
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.5847193829281233No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.43074822562154264No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10620.39401775677009326No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.23485239174714598No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG5460.2025741009382965Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT5290.19626684871127997Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT4400.16324652822866387No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG3890.1443247715476142No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT3780.14024360834189759No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA2800.10388415432733154No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAA2710.1005450207953816No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGAA253.886429E-544.9929518
AGCCTTC207.0290884E-444.9929512
CCGGAAT250.002105872835.9943583
GCCTTCT300.005144290629.99529813
AATCAGT300.005144290629.99529816
CGAGACT855.0154576E-621.17315323
AGGCATG754.571738E-521.00840430
TAGGCAT754.571738E-521.00840429
CCCCCAC654.220524E-420.7736851
TTAGGAG654.2311134E-420.7659765
CTCTCCA550.003919745320.4627328
ATTCAGG550.003923963320.4589311
AGGGCTG550.003932410420.4513444
GGCATGA807.512448E-519.6953831
GTATGCC807.544682E-519.68441642
GGGGCTC807.544682E-519.68441616
ATGATCT706.9457636E-419.29343234
GCATGAT706.9457636E-419.29343232
CTTGCCG600.006503751518.747069
CTTAGGA600.006503751518.747064
Produced by FastQC (version 0.11.2)