FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell65.351000000957bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell65.351000000957bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296471
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA13030.1005035978436849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC35750.023.662493
CCGATCT36350.023.210024
TTCCGAT36750.023.01952
CTTCCGA37200.022.6945611
CGATCTG14350.022.5766355
CGATCTT10050.020.3715445
CGATCTA5400.019.998445
CGATCTC10500.019.9270175
TCGTATA600.00651241918.749262
GTATAAC1507.215931E-918.0104031
CGCGCTA750.001110334117.99998341
GTATTAG3950.017.6684341
CGGTCCG1853.074092E-1017.02701239
ATTCGTA3750.016.20061124
CGCTAGA3500.016.07203537
ATTAGAC1552.0684638E-715.9664963
GCATTCG3750.015.59998622
TTCGTAT3900.015.5775125
TACGACG1602.9664443E-715.4675445
TTAGACT1754.8692527E-815.4273694
Produced by FastQC (version 0.11.2)