FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell64.35100000095794.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell64.35100000095794.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808066
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA8190.1013531072956912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG709.2995833E-725.71499335
CGGTACC751.6971153E-623.99917645
CGATCTG10650.023.0273765
CCGATCT26000.021.5473374
TCCGATC26050.021.505983
CGTCGTA955.529364E-721.31505610
GTATTAG2750.021.2851681
TTCCGAT26700.021.0679952
CTTCCGA26900.020.8393171
CGATCTT6500.020.7685175
GGCGTTA1251.7660568E-819.7993242
TACCGTC1151.5944534E-719.5645457
CCGTCGT1051.4449579E-619.2850519
GCGTTAT1454.7675712E-918.6200543
TTAGGAC1951.8189894E-1218.4609033
GCGCGCT750.001109630818.00049624
CTCGGTA1002.044929E-518.00049427
CGATCTA3700.017.634535
ATACCGT1305.7794205E-717.3070966
GTCGCCT1205.170965E-616.87441839
Produced by FastQC (version 0.11.2)