FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell63.3510000009577a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell63.3510000009577a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468809
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA47280.3218934524502505No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC26770.18225650850450945No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT24810.16891236369058196No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG23720.16149138519712228No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA19930.13568816639876255No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT19860.1352115897982651No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT17030.11594427866386985No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC16810.11444646649087799No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA16260.11070193605839833No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTA15640.10648082902542128No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC14740.10035341559045458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG501.9822546E-631.49971213
CGCATCG1152.6557245E-1023.47804813
CTGTGCG908.254459E-619.9998199
CGATCTG23150.019.7298435
ATAACGC1603.8198777E-1119.687323
TCGCCGG1403.088644E-919.2855417
ATCCGTA1051.446253E-619.2855412
AACGCCG1403.088644E-919.285545
CTTACCG706.985161E-419.285549
ATAGCGG1555.347829E-1018.8707967
TCTACGC600.006511679418.7498283
CATCGTT1454.776666E-918.6205228
GCATCGC1454.776666E-918.6205214
TACTCGC851.2048139E-418.52924520
TAACGCC1608.1672624E-1018.2810844
GTATTAT3350.018.1415751
GCATCGT1507.2705006E-917.99983827
CGGTCGG2001.8189894E-1217.99983621
CCGTTTA1406.480332E-817.67841113
GCGTATA1153.4502846E-617.6085369
Produced by FastQC (version 0.11.2)