FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell62.35100000095751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell62.35100000095751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289232
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA450.001226974524.99881616
ACGTCGT551.3680483E-424.54619636
ACCGTAC551.3687687E-424.54429217
CGATCTG14950.022.1235565
CGATCTA5300.021.6507575
TCCGATC37350.021.5660783
CCGATCT37550.021.4512144
TTCCGAT38100.021.0824952
CTTCCGA39250.020.6519831
CGATCTC11450.017.4889325
CGATCTT9550.016.9632035
TTGCGGT1602.96277E-715.46921635
TAATACC1907.998096E-915.3946044
GCATAGG900.00373609715.0109331
GGTACTA1209.754331E-514.99987239
GTATTAG4450.014.6736091
GTATAGA2152.5556801E-914.661841
ATAACTA1705.889815E-714.5586985
GATCTGG8400.014.4641616
TATACTG2801.8189894E-1214.4641615
Produced by FastQC (version 0.11.2)