FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell61.35100000095737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell61.35100000095737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003707
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA72000.2397038059970563No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC47440.1579381743958382No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT41220.13723042893331472No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA37130.12361392106487083No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG37120.12358062886959346No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT35290.11748815713383495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG33450.025.1559945
TTCCGAT79950.023.3011762
TCCGATC80950.023.1245043
CTTCCGA81750.022.9123211
CCGATCT81800.022.80174
CGGTACC5150.021.40695245
CGATCTA11950.021.0870655
CGATCTT22750.020.2739545
TTAACGG6200.018.50828435
GTATTAG14550.018.2579731
ATCTGCG4300.017.2667837
ACCGCCT7150.016.36301212
CGATCTC20200.016.0389925
CGGTACT8800.015.59677538
TAACGGC7350.015.30630336
TCTTCCG42950.015.2438895
CGTCGTA3400.015.22000810
GTATATA12050.014.9463951
TACCGTC3350.014.7755577
GGTACTA9800.014.69405239
Produced by FastQC (version 0.11.2)