FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell60.35100000095700.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell60.35100000095700.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657874
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG26600.021.8222875
TCCGATC67400.020.9967923
TTCCGAT67850.020.8910872
CCGATCT67800.020.8729174
CTTCCGA68550.020.7903041
CGATCTT17850.019.788995
CGATCTA11750.019.7224835
CGCGAAG4200.017.6784122
GTATTAG9750.017.0880181
CCCGCGA4200.016.07128120
CCGTCGG4250.015.88250540
CCGCGAA4650.015.48372821
CGTTATT3500.015.42755844
GGCGTTA3650.015.41052842
GGTACTA3350.014.77626139
TTAACGG2456.91216E-1114.69429635
CCCGTCG4800.014.53138939
CTATCGC2203.5743142E-914.31885830
CTTATCG1108.590498E-414.31885834
CGATCTC18600.014.2735255
Produced by FastQC (version 0.11.2)