FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell6.35100000095058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell6.35100000095058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675962
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA46810.17492774561073737No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC27400.10239308330985267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG20300.028.5958965
TCCGATC51400.027.2712443
CTTCCGA51450.027.2223721
TTCCGAT51650.027.1402572
CGATCTA6700.026.8655155
CCGATCT52400.026.664924
CGATCTT16600.020.4667475
CGATCTC15300.019.7057695
TCTTCCG22450.019.5433165
CGCTAGA4150.018.97543737
GTAATCG1555.347829E-1018.87050420
GCTCTTC24650.017.6165313
CCGCTAG4500.016.99958436
GGTACTA5100.016.76429639
CGGTACT5300.016.1316838
GATCTCG6350.015.5904626
GAATCGG1451.8113751E-615.51686222
ATCGATA1754.8727088E-815.42819423
GCATTCG5000.015.29962522
GTATTAA8850.015.26612951
Produced by FastQC (version 0.11.2)