FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell59.351000000956e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell59.351000000956e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857718
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22920.2672206949137129No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG13720.1599593339535838No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC13280.1548294427772298No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT11490.13396011276433514No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA10140.11822067392779445No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC9330.10877701062587004No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT9070.10574571129438814No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT8800.10259782352708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTA300.00514764929.99867812
TAGCGAG608.187477E-626.25037228
TTAGCGA651.5229967E-524.23111527
GCGAGGT702.702879E-522.4990121
CGGTACC1800.021.24906345
CGAGCAT754.600378E-521.000337
ACGAGCA754.600378E-521.000336
GACGATT654.238281E-420.76831614
CGCGGTA1850.020.67476743
TTGCGGT1750.020.57172435
CGAAGAT550.003936739620.45364644
GACGTAG1009.048181E-720.25028825
AGACGTA1009.048181E-720.25028824
CGTAGAC1009.048181E-720.25028827
CGGTACT1800.020.00028438
CTTGCGG1850.019.45973834
CGAGAGG1051.4444959E-619.2859923
ACGTAGA1051.4444959E-619.2859926
CGATTAG706.98356E-419.28486616
CGCACTA600.00650864718.75026527
Produced by FastQC (version 0.11.2)