FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell58.351000000956cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell58.351000000956cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326947
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19610.14778284287164445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA207.0327224E-445.00124441
TCGACGG300.005147794329.999744
CGATCTG13100.023.8729065
TCCGATC32600.022.9820333
CCGATCT33000.022.7034634
TTCCGAT33750.022.1989422
CTTCCGA34350.021.8218771
CGATCTT8350.021.016965
CGATCTA5650.017.9194985
GTATTAG4350.017.5939871
TAACGCC1953.45608E-1117.3068664
ATAATAC4200.017.142043
CGATCTC9500.017.0518195
ATAACGC2403.6379788E-1215.9367413
CGATTTC1703.405512E-815.88279242
CGAGCGT850.002566261715.8815966
GCTACGA1004.1691578E-415.7492492
GTATAAT4450.015.6810891
TACCGTC1908.003553E-915.3940027
GGCGTTA1654.1998828E-715.00041642
Produced by FastQC (version 0.11.2)