FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell57.351000000956a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell57.351000000956a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301588
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24910.1082296223303215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA10150.024.6046895
TCCGATC64550.021.0872553
TTCCGAT65300.020.8799652
CCGATCT65400.020.744384
CTTCCGA66900.020.427541
CGATCTG25800.020.057145
CGATCTT16850.018.4263845
GCATTCG6050.017.10695822
GTATTAG7400.016.4291671
CGGTCGA1104.6427413E-516.36282220
ATTCGTA6350.016.29946524
GGTACTA3100.015.96833539
CGCTAGA6500.015.23133637
GATCTAG5350.015.1394346
CGGTACT3300.015.00055838
CTAGCGT900.003756687314.9992544
TATCCCG1056.042399E-414.9992535
GTATAAT7700.014.9118991
CCGCTAG6800.014.8902636
ATAATAC7750.014.8057163
Produced by FastQC (version 0.11.2)