FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell56.35100000095688.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell56.35100000095688.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771284
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA2050.029.6355135
TTCCGAT15400.027.1765772
TCCGATC15450.027.0886273
CTTCCGA15650.026.8984321
CCGATCT15600.026.6839224
TATACCG450.001225859525.0011525
CGATCTG6550.024.3904385
CGATCTT3900.023.6549365
CGATCTC5350.019.3466875
TAGGACA1051.44367E-619.2866044
TCGCCTA600.006509301718.7496533
CCGGTAG600.006509301718.749659
TCGGGTG1454.7657522E-918.6203415
ATTATAC2100.018.2151263
GTATTAA2550.017.6558861
ACACTAT901.8640161E-417.5008076
CGAGGGT901.8649406E-417.49967216
ATAACTC800.001709629116.8757783
GGCGATA1257.6209963E-616.19969723
CGCCACT1401.2536311E-616.07112728
Produced by FastQC (version 0.11.2)