FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell55.3510000009566e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell55.3510000009566e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799605
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA12380.154826445557494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT5150.025.3389265
CGTGCGT602.466134E-422.50062637
CGATCTA2650.022.0747035
TCCGATC20650.021.8999653
CCGATCT20650.021.8999654
CGGTACT1551.8189894E-1221.77479738
TTCCGAT20700.021.7383732
CTTCCGA21150.021.5034071
GCGTACT654.2358815E-420.76980840
GCCGTTA1109.978248E-820.45383515
CGATCTG8850.020.3382765
CCGTTAA1009.0508547E-720.24929616
CGACTTT1503.43789E-1019.49932124
CCTTGCG1757.2759576E-1219.28625133
TGCGTAC706.979618E-419.28625139
TTGCGGT1851.6370905E-1118.2437535
GCGGTAC2001.8189894E-1218.00050237
TCGTTCT1751.382432E-1018.000530
TTCGTTC1751.382432E-1018.000529
GTGCGTA750.001109614918.000538
Produced by FastQC (version 0.11.2)