FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell54.35100000095645.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell54.35100000095645.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845125
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA43330.1522955933394842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG31300.023.1459185
TCCGATC79900.022.55533
TTCCGAT80150.022.5130182
CTTCCGA81150.022.2742771
CCGATCT80900.022.1930624
CGATCTA13100.021.4684935
CGATCTT19150.020.4429385
CTGCGTA807.570642E-519.686619
CGATCTC24800.016.6020725
CCCCTAT17750.015.5985181
TTAACGG2353.6379788E-1115.31980135
TACGCGC900.003754232515.00090228
GGCGTTA2950.014.49137842
ATAATAC9650.014.4553043
GTATTAA8100.014.45091
GCATTCG6750.014.33293522
ACGTCCG1108.5956376E-414.31778443
CGCTCTA950.005380208614.20988414
GTATTAG10100.014.0409271
CGGTACT5450.014.03655538
Produced by FastQC (version 0.11.2)