FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell53.35100000095638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell53.35100000095638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362622
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23850.023.86775
CCGATCT61800.022.0628994
TCCGATC61750.022.0078893
TTCCGAT62500.021.8522572
CTTCCGA62950.021.7818221
CGATCTA11000.021.0679845
CGATCTT16300.018.2206865
ACCGTAC800.001711917116.87448517
ACGAAAC1104.6420613E-516.36313616
GGCGTTA2650.016.13158242
GTATTAA6550.015.8120861
CGATCTC17800.015.8004145
GTCTATA4050.015.0100251
GTATAGA3900.015.0100251
ACGCCGT900.003755191315.00017534
GTATATA7150.014.8000941
ATCTGCG3300.014.3177447
GTTATAC2853.6379788E-1214.2200231
CCGTTAA1758.1819053E-714.14242516
TATCCCG1453.1073607E-513.9653865
Produced by FastQC (version 0.11.2)