FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell52.3510000009561e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell52.3510000009561e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077412
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG22850.022.4505255
TCCGATC58300.020.7630713
TTCCGAT58200.020.7600882
CCGATCT58650.020.5240764
CTTCCGA59300.020.5026361
CGATCTT15450.020.0968745
ATACCGA1354.2775355E-818.3331366
CGGGTAT1153.4525256E-617.60808215
GTATATA6050.017.1187921
CGATCTA8950.016.0892125
CGTCGTA2850.015.78892410
ATCTGCG2850.015.7889247
CGATTAC1004.1701627E-415.74945345
TACCGTC3350.015.4472257
ACGACGG2950.015.2540746
TAGTACG1502.5762165E-614.9998394
TATCGAT1056.041364E-414.99947842
GTATTAG6950.014.9019691
TACGACG2900.014.741225
CGCATCG2801.8189894E-1214.46378213
Produced by FastQC (version 0.11.2)