FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell51.351000000955f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell51.351000000955f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144764
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23200.020.8511855
TCCGATC63050.019.4844363
CCGATCT63050.019.448754
TTCCGAT63950.019.2810382
CTTCCGA65100.019.1230561
CGATCTA10500.018.4284765
CGATCTT15750.018.285625
GTATATA7300.017.5778271
GTATTAG6900.016.965531
ATCGATT2101.07320375E-1016.07134643
ATATCGC1156.7871835E-515.6520936
TAATACG900.003755463314.9999224
TCGTTAA3800.014.80221112
CGATTAC1859.628275E-814.59417845
TATCGAT1901.3337194E-714.21045342
CTCGTTA3800.014.21012211
GTCCTAT6200.014.1607461
GATCTGT9700.014.1494116
CGTCGTA1758.1806866E-714.14245410
GTACTAG1605.0034487E-614.0699721
Produced by FastQC (version 0.11.2)