FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell50.351000000955d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell50.351000000955d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3412682
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA74790.2191531469969953No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC45310.13276947573785075No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT37880.11099774312402973No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT36180.10601632381804105No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG35870.10510794735636077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT33550.018.7774125
CGGGTAT2150.017.78993215
CGGTACC4900.016.0707445
CCGATCT149650.016.011914
TCCGATC150900.015.9538233
TAGGGGT17350.015.9505574
TTCCGAT152250.015.842382
GGCGTTA4150.015.72221742
CGATCTG58900.015.6998945
GGGGTTA16250.015.5072546
CGTCGTA3350.015.44709810
TACCGTC3650.015.4102967
CTTCCGA159850.015.3249261
TTAGCGA1056.03912E-415.00067327
GATCTTT25200.014.9995756
ATAGGGG19000.014.92062953
GGGTTAG17700.014.6180157
ACGCTAT6050.014.503509510
ACGTCCG1251.3822185E-414.39938243
AGGGGTT17900.014.3292045
Produced by FastQC (version 0.11.2)