FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell5.3510000009503e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell5.3510000009503e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090203
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA34050.16290283766696345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG17200.027.6003515
TCCGATC42400.027.5929473
TTCCGAT42550.027.3370442
CCGATCT42750.027.2617824
CTTCCGA43050.027.1409441
CGATCTA5500.026.9986365
CGATCTT13100.023.35765
TCGGTAA550.00393602420.4559627
CGATCTC12700.019.3100475
TACCGTC1656.002665E-1119.0899457
CGTAGCG1555.347829E-1018.87001415
TCTTCCG19100.018.258245
TACCGAT750.001111013117.9990927
ATCTGCG2200.017.3854877
ATACCGT2500.017.0991366
ACCGTCG1904.4929038E-1016.578118
CGGGTAC2251.8189894E-1115.9991917
ACGGGTA850.002566972515.8815516
GCTCTTC21750.015.8267863
CGTCGTA1855.631591E-915.81001210
Produced by FastQC (version 0.11.2)