HT5LJBCXY_l01n01_mock_cell49.351000000955bf.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HT5LJBCXY_l01n01_mock_cell49.351000000955bf.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387104
Sequences flagged as poor quality	0
Sequence length	51
%GC	51

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1623	0.4192671736794247	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1266	0.32704389518062327	No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1095	0.28286971976523106	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	983	0.25393692651070515	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT	894	0.23094568901380505	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG	734	0.1896131272216252	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT	676	0.17463007357196	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACG	657	0.16972183185913864	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT	655	0.16920517483673636	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA	573	0.1480222369182442	No Hit
GCCCAGGGAGACGTGTGCTCTTCCGATCTGCCCAGGGAGACGTGTGCTCTT	406	0.10488137554765643	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCAC	30	0.0051448233	29.997932	45
AGGGCTC	30	0.0051448233	29.997932	15
GAATCTC	40	6.1565265E-4	28.126694	32
TTCTGGA	45	0.0012246544	25.001509	30
TCTATTG	45	0.0012246544	25.001509	29
CCAACCC	45	0.0012255772	24.99828	43
GCCGTCT	45	0.0012255772	24.99828	43
TACGTCT	45	0.0012255772	24.99828	4
CTCGTAT	50	0.0022611257	22.501356	36
TCGTATG	50	0.0022611257	22.501356	37
GTATAGG	55	0.003924642	20.461065	1
GCGAATC	55	0.003930522	20.455778	30
GGCGAAT	55	0.003930522	20.455778	29
TGCCGTC	55	0.003930522	20.455778	42
CATACGT	55	0.0039334646	20.453136	2
TCTTACT	70	6.975259E-4	19.284386	8
AGACTAA	60	0.0065006143	18.751131	22
GTATGCC	60	0.0065006143	18.751131	39
AATCTCG	60	0.0065006143	18.751131	33
GACTAAG	60	0.0065006143	18.751131	23